Flow cytometric analysis of Human CD4 expression on human PBMC (Human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 488 Mouse Anti-Human CD3 antibody and either Alexa Fluor® 647 Rabbit IgG Isotype Control (Left panel) or SDT Alexa Fluor® 647 Rabbit Anti-Human CD4 Antibody (Right panel) at 1 μg/test. Fluorescent histograms were gated events with lymphocytes. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rabbit Anti-Human CD4 Antibody (S-R163)
Alexa Fluor® 647 Rabbit Anti-Human CD4 Antibody (S-R163)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD4 |
| Synonyms | T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4 |
| Location | Cell membrane |
| Accession | P01730 |
| Clone Number | S-R163 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.2mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume |
Background
CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells. It is a type of white blood cell that helps fight infection by triggering your immune system to destroy viruses, bacteria, and other germs that may make you sick. CD4 is also a receptor for the HIV virus, and when the virus infects cells with CD4 surface proteins, it depletes the number of T cells, B cells, natural killer cells, and monocytes in the patient's blood.
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