Alexa Fluor® 488 Mouse Anti-Human Integrin beta1/CD29 Antibody (S-933-228)
Alexa Fluor® 488 Mouse Anti-Human Integrin beta1/CD29 Antibody (S-933-228)
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$110 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Integrin beta1/CD29 |
| Synonyms | Fibronectin receptor subunit beta; Glycoprotein IIa (GPIIA); VLA-4 subunit beta; ITGB1; FNRB; MDF2; MSK12 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P05556 |
| Clone Number | S-933-228 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein G |
| Concentration | 1mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu |
Background
CD29 is an integrin component that mediates adhesion and involves in homing to sites of inflammation. It expresses in fibroblasts, platelets, T cells, monocytes, granulocytes(low), mast cells, endothelial cells and myoepithelium, also other diverse cell types. It does not express in red blood cells and spermatogonia. It is a myoepithelial marker, although established markers (SMA, CD10, p63, S100, maspin, calponin, GFAP, smooth muscle myosin) are more commonly used.
Picture
Picture
FC
Flow cytometric analysis of Human Integrin beta1/CD29 expression on human PBMC (human peripheral blood mononuclear cell). Cells Fluorescent histograms were gated events with monocytes (Right) or lymphocytes (Left) was stained with either Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Human Integrin beta1/CD29 Antibody (Red line histogram) at 5 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
