Flow cytometric analysis of Human CD28 expression on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Mouse IgG1 Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD28 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse Anti-Human CD28 Antibody (S-R547)
Alexa Fluor® 488 Mouse Anti-Human CD28 Antibody (S-R547)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD28 |
| Synonyms | T-cell-specific surface glycoprotein CD28; TP44 |
| Location | Cell membrane |
| Accession | P10747 |
| Clone Number | S-R547 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood lymphocytes |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu |
Background
CD28, also known as the T-cell-specific surface glycoprotein CD28, is a protein encoded by the CD28 gene in humans. It is a glycoprotein that is constitutively expressed on both resting and activated T cells. CD28 plays a crucial role in T cell activation and signaling, providing essential co-stimulatory signals required for T cell activation, proliferation, and cytokine production. It enhances the production of IL4 and IL10 in T cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. CD28 interacts with its ligands, CD80 (B7-1) and CD86 (B7-2), which are expressed on antigen-presenting cells, to mediate the co-stimulatory signals necessary for T cell activation. This interaction is critical for the early events of T cell activation and the decision between immunity and anergy.
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