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AGXT Recombinant Rabbit mAb (S-3586-30)

AGXT Recombinant Rabbit mAb (S-3586-30)

Catalog Number: S0B6702 Application: WB, IHC-P Reactivity: Human,Mouse, Rat Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen AGXT
Synonyms Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase (SPT); AGT1; SPAT; SPYA
Immunogen Recombinant Protein
Location Peroxisome
Accession P21549
Clone Number S-3586-30
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu, Ms, Rt
Positive Sample HepG2, mouse liver, rat liver
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu, Ms, Rt
IHC-P 1:500 Hu

Background

The AGXT protein (SPYA) is a potent superantigen produced primarily by Streptococcus pyogenes (Group A Streptococcus), playing a critical role in the pathogenesis of severe invasive infections such as streptococcal toxic shock syndrome (STSS) and necrotizing fasciitis. As a member of the pyrogenic toxin superantigen (PTSAg) family, AGXT functions by bypassing normal antigen presentation mechanisms, binding simultaneously to major histocompatibility complex class II (MHC II) molecules on antigen-presenting cells and specific Vβ regions of the T-cell receptor, resulting in massive polyclonal T-cell activation (up to 20-30% of the total T-cell repertoire), cytokine storm production (particularly TNF-α, IL-1β, and IL-6), and subsequent systemic inflammation, capillary leakage, and multi-organ failure. Structurally, SPYA consists of two distinct domains: an N-terminal oligosaccharide/oligonucleotide-binding (OB) fold domain responsible for MHC II binding and a C-terminal β-grasp domain that interacts with the T-cell receptor, with the protein's stability and activity influenced by zinc-binding sites and disulfide bonds. The speA gene encoding AGXT is typically located on bacteriophage genomes, facilitating horizontal gene transfer among streptococcal strains and contributing to the emergence of highly virulent clones. Clinical isolates from severe infections frequently harbor the speA gene, and serological studies indicate that antibody levels against SPYA correlate with disease severity, though protective immunity remains strain-specific due to significant allelic variation among AGXT variants (SPEA1-4). Therapeutic strategies targeting AGXT include neutralizing monoclonal antibodies and peptide inhibitors that disrupt the MHC II-TCR bridging interface, while current research also explores the structural basis of superantigen activity to develop broad-spectrum countermeasures against this and related toxins.

Picture

Western Blot

WB result of AGXT Recombinant Rabbit mAb
Primary antibody: AGXT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 38 kDa
This blot was developed with high sensitivity substrate

WB result of AGXT Recombinant Rabbit mAb
Primary antibody: AGXT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 38 kDa

WB result of AGXT Recombinant Rabbit mAb
Primary antibody: AGXT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 38 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-AGXT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-AGXT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-AGXT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human ovarian cancer. Anti-AGXT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-AGXT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-AGXT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Expression Atlas

Expression of AGXT in tumor tissues.

Expression of AGXT in hepatic cancer tissues.

Expression of AGXT in human tissues.