WB result of ACLY/ATP citrate lyase Recombinant Rabbit mAb
Primary antibody: ACLY/ATP citrate lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 115 kDa
ACLY/ATP citrate lyase Recombinant Rabbit mAb (S-2025-32)
ACLY/ATP citrate lyase Recombinant Rabbit mAb (S-2025-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ACLY/ATP citrate lyase |
| Synonyms | ATP-citrate synthase; ATP-citrate (pro-S-)-lyase (ACL); Citrate cleavage enzyme |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P53396 |
| Clone Number | S-2025-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, MCF7, HepG2, Jurkat, 293T, NIH/3T3, mouse liver, C6, rat liver |
| Predicted Reactivity | Sh, Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
ACLY, also known as ATP citrate lyase, is a crucial enzyme involved in cellular metabolism. It catalyzes the conversion of citrate and ATP to oxaloacetate, acetyl-CoA, and ADP, playing a key role in linking glycolysis and lipid metabolism. ACLY is predominantly located in the cytoplasm and is essential for the production of acetyl-CoA, which serves as a vital precursor for fatty acid synthesis and cholesterol biosynthesis. Its activity is tightly regulated by various factors, including insulin and AMPK, reflecting its importance in maintaining metabolic homeostasis. Abnormal expression or activity of ACLY has been implicated in several diseases, such as cancer and metabolic disorders, making it an attractive target for therapeutic interventions.
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Western Blot
WB result of ACLY/ATP citrate lyase Recombinant Rabbit mAb
Primary antibody: ACLY/ATP citrate lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 115 kDa
WB result of ACLY/ATP citrate lyase Recombinant Rabbit mAb
Primary antibody: ACLY/ATP citrate lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 115 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-ACLY/ATP citrate lyase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
