All AntBio Solutions

  • Conventional Mammalian Adherent Cells
  • Mammalian Suspension Cells
  • Mammalian Soft Tissues
  • Animal Blood Proteins
  • Bone Tissues
  • Bacteria
  • Yeast
  • Insect Cells
  • Plant Tissues
  • Membrane Proteins
  • Organelles/Suborganelles
  • Nuclear/Cytoplasmic Protein Extraction
  • Mitochondrial/Cytoplasmic Fraction Extraction
  • Endoplasmic Reticulum Extraction
  • Exosome Extraction
  • Protein Extraction from FFPE Samples
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    Solution

    WB

    The process involves separating proteins of different molecular weights via polyacrylamide gel electrophoresis (PAGE), transferring them onto a solid support (such as a PVDF membrane or nitrocellulose (NC) membrane), and then detecting the target protein using antigen-antibody reactions. Throughout the entire experimental process, every step—from sample preparation to antibody incubation—plays a crucial role in determining the experimental results.

    WB

    ANT Bio Solution

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    WP

    This manual provides detailed descriptions of the operating methods and precautions for each step, following the experimental workflow of Western blotting. In addition, it summarizes various practical tips for the experiment for reference.

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    ANT Bio Solution

      1. Sample Preparation

      1. Sample Preparation
      1.1 Sample Types
      1.1.1 Conventional mammalian adherent cells
      1.1.2 Mammalian suspension cells


      1.1.3 Mammalian soft tissues


      1.1.4 Animal blood proteins


      1.1.5 Bone tissues


      1.1.6 Bacteria


      1.1.7 Yeast


      1.1.8 Insect cells


      1.1.9 Plant tissues


      1.1.10 Membrane proteins


      1.1.11 Organelles / subcellular components


      1.1.12 Nuclear / cytoplasmic protein extraction


      1.1.13 Mitochondria / cytoplasmic fraction extraction


      1.1.14 Endoplasmic reticulum extraction


      1.1.15 Exosome extraction


      1.1.16 Protein extraction from FFPE samples



      • 1.2 Protein Quantification


        1.2.1 UV spectrophotometry


        1.2.2 BCA assay


        1.2.3 Coomassie brilliant blue assay



      • 1.3 Protein Denaturation

      1. Sample Preparation

      1. Sample Preparation


      • 1.1 Sample Types


        1.1.1 Conventional mammalian adherent cells


        1.1.2 Mammalian suspension cells


        1.1.3 Mammalian soft tissues


        1.1.4 Animal blood proteins


        1.1.5 Bone tissues


        1.1.6 Bacteria


        1.1.7 Yeast


        1.1.8 Insect cells


        1.1.9 Plant tissues


        1.1.10 Membrane proteins


        1.1.11 Organelles / subcellular components


        1.1.12 Nuclear / cytoplasmic protein extraction


        1.1.13 Mitochondria / cytoplasmic fraction extraction


        1.1.14 Endoplasmic reticulum extraction


        1.1.15 Exosome extraction


        1.1.16 Protein extraction from FFPE samples



      • 1.2 Protein Quantification


        1.2.1 UV spectrophotometry


        1.2.2 BCA assay


        1.2.3 Coomassie brilliant blue assay



      • 1.3 Protein Denaturation

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