After 5 days of RANK L induction, tartrate-resistant acid phosphatase (TRAP) staining was performed to specifically identify osteoclasts. After the staining was completed, it was observed under an optical microscope. The cytoplasm of mature osteoclasts appeared red or red-purple.
TRANCE/RANK L/TNFSF11 (159aa) Protein, Mouse(RAW264.7破骨专用）
TRANCE/RANK L/TNFSF11 (159aa) Protein, Mouse(RAW264.7破骨专用）
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor (ODF); Osteoprotegerin ligand (OPGL); Receptor activator of nuclear factor kappa-B ligand (RANKL); TNF-related activation-induced cytokine (TRANCE); CD254 |
| Accession | O35235 |
| Amino Acid Sequence | Lys158-Asp316, with N-terminal His tag and Flag tag. |
| Expression System | HEK293 |
| Molecular Weight | 20-25 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag, Flag Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Nat Rev Drug Discov. 2024 Dec;23(12):939-961. |
Background
TNFSF11, also known as Receptor Activator of Nuclear Factor-κB Ligand (RANKL), is a crucial cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is expressed on osteoblasts or T cells and binds to TNFRSF11B (OPG) and TNFRSF11A (RANK) on osteoclasts and precursor cells to regulate their differentiation and proliferation. RANKL plays a vital role in bone remodeling by stimulating osteoclast activity and is essential for maintaining bone homeostasis. In mice, the TNFSF11 gene is located on chromosome 14 and encodes a glycoprotein with an extracellular domain that can be cleaved and released into the bloodstream.
TNFSF11 is a key regulator of osteoclastogenesis and bone resorption. In bone organoid models, TNFSF11 signaling is essential for maintaining the balance between osteoblasts and osteoclasts. By manipulating TNFSF11 levels, researchers can study bone diseases such as osteoporosis and osteopetrosis in a more physiologically relevant context.
TNFSF11 is involved in the development and function of secondary lymphoid organs. In immune organoids, TNFSF11 signaling can influence the formation of lymphoid structures and the activation of immune cells. This makes it a valuable target for studying immune responses and developing new therapeutic strategies for autoimmune diseases.
Guidelines
Recommended dosage for osteoclast differentiation RANKL: 100 ng/mL; The recommended dosage of M-CSF (product number: UA040128) is 50 ng/mL.
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Bioactivity
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is less than 5ng/ml.
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
>95% as determined by SEC-HPLC.
