Measured in a cell proliferation assay using M-07e human megakaryocytic leukemic cells. The EC50 for this effect is less than 1ng/ml.
TPO (332aa) Protein, Human
TPO (332aa) Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Thrombopoietin; C-mpl ligand (ML); Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor (MGDF); Myeloproliferative leukemia virus oncogene ligand; |
| Accession | P40225 |
| Amino Acid Sequence | Ser22-Gly353 with C terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | 80-90 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Nature. 1994 Jun 16;369(6481):533-8. |
Background
Thrombopoietin (TPO) is a glycoprotein hormone that plays a critical role in the regulation of platelet production and megakaryopoiesis, the process by which megakaryocytes give rise to platelets. TPO acts on hematopoietic stem cells and progenitor cells in the bone marrow, stimulating their differentiation into megakaryocytes and promoting platelet formation. TPO exerts its effects by binding to the c-Mpl receptor on the surface of hematopoietic cells, leading to the activation of signaling pathways that regulate cell proliferation, survival, and differentiation. Dysregulation of TPO signaling has been implicated in various hematological disorders, such as thrombocytopenia and myeloproliferative neoplasms.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
