Product Details
Product Details
Product Specification
Species | Human |
Synonyms | TPK1, Thiamin pyrophosphokinase 1, hTPK1, Placental protein 20, PP20, Thiamine pyrophosphokinase 1 |
Accession | Q9H3S4 |
Amino Acid Sequence | Met1-Ser243, with C-8*His |
Expression System | E.coli |
Molecular Weight | 27-28kDa |
Purity | >95% by SDS-PAGE |
Endotoxin | <1EU/μg |
Conjugation | Unconjugated |
Tag | His Tag |
Physical Appearance | Lyophilized Powder |
Storage Buffer | PBS, pH7.4 |
Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Reference |
1、Mayr J A. et al. (2011) Thiamine pyrophosphokinase deficiency in encephalopathic children with defects in the pyruvate oxidation pathway. Am J Hum Genet. 89(6): 806-812. 2、Bellyei S.et al. (2005) Cloning, sequencing, structural and molecular biological characterization of placental protein 20 (PP20)/human thiamin pyrophosphokinase (hTPK). Placenta. 26(1): 34-46. |
Background
Thiamin pyrophosphokinase 1 (TPK1), a member of the thiamine pyrophosphokinase family, is also known as Thiamine pyrophosphokinase 1, Placental protein 20 (PP20) and hTPK1, which is detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues. TPK1 is regarded as a Kinase and a Transferase that can bind ATP and Nucleotide.
Picture
Picture
SDS-PAGE

1μg (R: reducing condition, N: non-reducing condition).
