WB result of Phospho-TAK1 (Ser412) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-TAK1 (Ser412) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Calyculin A for 10 minutes and 20 ng/ml human IL-1ß for 10 minutes whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 70 kDa
Phospho-TAK1 (Ser412) Recombinant Rabbit mAb (S-3334-138)
Phospho-TAK1 (Ser412) Recombinant Rabbit mAb (S-3334-138)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-TAK1 (Ser412) |
| Synonyms | Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1 (TGF-beta-activated kinase 1); TAK1; MAP3K7 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | O43318 |
| Clone Number | S-3334-138 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa (treated with 100 nM Calyculin A for 10 minutes and 20 ng/ml human IL-1ß for 10 minutes), K562, C2C12, C6, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
Phospho-TAK1 (Ser412) is the activated form of transforming growth factor-β-activated kinase 1 (TAK1, also known as MAP3K7) following phosphorylation at serine residue 412, representing the activation state of this serine/threonine kinase signaling pathway. Ser412 is located within the activation loop region of TAK1, and its phosphorylation is critical for TAK1 kinase activity. When cells are stimulated by inflammatory cytokines (such as IL-1β, TNF-α), TGF-β, or various stress signals, TAK1 is activated through ubiquitination-dependent mechanisms. Phosphorylation at Ser412 promotes the interaction of TAK1 with its cofactors TAB1/TAB2/TAB3, subsequently activating downstream NF-κB and MAPK (including JNK and p38) signaling pathways, thereby regulating diverse biological processes such as inflammatory responses, apoptosis, autophagy, and fibrosis. Studies have shown that aberrant phosphorylation at Ser412 is closely associated with sustained activation of TAK1 in autoimmune diseases, inflammatory bowel disease, liver fibrosis, and various cancers.
Picture
Picture
Western Blot
WB result of Phospho-TAK1 (Ser412) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-TAK1 (Ser412) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 70 kDa
WB result of Phospho-TAK1 (Ser412) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-TAK1 (Ser412) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 70 kDa
WB result of Phospho-TAK1 (Ser412) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-TAK1 (Ser412) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 70 kDa
