Human IL-6 Kit (HICA)
Human IL-6 Kit (HICA)
Product Details
Product Details
Product Specification
| Stability & Storage | Store at 2~8°C protected from light for 12 months; the reconstituted standard can be aliquoted and stored at -20°C, avoiding repeated freeze-thaw cycles. |
Background
Test Principle:
This kit employs a homogeneous immuno chemiluminescence assay (HICA) based on the dual-antibody sandwich method for the detection of cytokine concentrations. The operation is simple and requires no washing steps.
The detection system consists of two types of microspheres: acceptor microspheres conjugated with antibody 1 targeting the protein of interest, and donor microspheres conjugated with streptavidin, along with biotin-labeled antibody 2. During the reaction, the target protein binds with the antibodies and microspheres to form an immune complex, bringing the two types of microspheres into close proximity. When the distance between the donor and acceptor microspheres is less than 200 nm, singlet oxygen generated upon light excitation can transfer to the acceptor microsphere and trigger chemiluminescence. Conversely, if the target protein is absent, the distance between the microspheres is too large, and no signal is produced.
By measuring the intensity of the chemiluminescent signal, quantitative analysis of the target protein can be achieved. This method features simple operation, rapid reaction, and high sensitivity.
Components
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Name |
Specification |
Component Specification |
Detection ReagentR1 |
500T |
2mL/bottle×1 |
2000T |
8ml/bottle×1 |
|
10000T |
40ml/bottle×1 |
|
Detection ReagentR2 |
500T |
2mL/bottle×1 |
2000T极地 |
8ml/bottle×1 |
|
10000T |
40ml/bottle×1 |
|
Detection ReagentR3 |
500T |
5mL/bottle×1 |
2000T |
20ml/bottle×1极地 |
|
10000T |
100ml/bottle×1 |
|
Standard |
500T |
0.015μg lyophilized product×1 |
2000T |
0.015μg lyophilized product×2 |
|
10000T |
0.015μg lyophilized product×5 |
|
Standard Buffer |
500T |
6mL/bottle×1 |
2000T |
12ml/bottle×1 |
|
10000T极地p> |
30ml/bottle×1 |
Note: The recommended plates for use are microplates (384 or 96-well plates, white, shallow wells)
```Guidelines
Reagent R3 must be protected from light during use. Sample addition and incubation should be performed under green light (<100 LUX) conditions.
Recalibration is required for each test, with at least duplicate wells for each standard concentration point. Use a four-parameter logistic curve fit (weighting: 1/Y²) for calculation.
Temperature and time must be strictly controlled during incubation. Microplates should be covered with a sealing film, and a microplate reader with ALPHA function is recommended.
The dilution matrix for calibrators should match that of the test samples. Reconstituted calibrators must be used within 2 hours.
Components from different reagent kit lots must not be mixed.
