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GluN3B Recombinant Rabbit mAb (S-2320-3)

GluN3B Recombinant Rabbit mAb (S-2320-3)

Catalog Number: S0B6805 Application: WB, ICC Reactivity: Human,Mouse Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen GluN3B
Synonyms Glutamate receptor ionotropic, NMDA 3B; GRIN3B
Immunogen Recombinant Protein
Location Cell membrane
Accession O60391
Clone Number S-2320-3
Antibody Type Recombinant mAb
Isotype IgG
Application WB, ICC
Reactivity Hu, Ms
Positive Sample CHO transfected with GluN3B-Myc-His fusion protein
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:500-1:1000 Ms
ICC 1:500 Ms

Background

GluN3B, encoded by the GRIN3B gene, is a unique and atypical subunit of the N-methyl-D-aspartate receptor (NMDAR) that fundamentally alters the receptor's functional properties when co-assembled with obligatory GluN1 subunits and conventional GluN2 subunits. Unlike other NMDAR subunits, GluN3B contains a distinct ligand-binding domain that renders the resulting heterotrimeric or heterotetrameric receptors insensitive to the co-agonist glycine and significantly reduces their calcium permeability and single-channel conductance, effectively acting as a dominant-negative modulator that dampens excitatory synaptic transmission. Primarily expressed in specific brain regions such as the thalamus, striatum, and during early postnatal development in the cortex, GluN3B plays a critical role in refining neural circuits by limiting excessive NMDAR activity, thereby influencing processes like synaptic plasticity, motor coordination, and sensory gating; furthermore, dysregulation or mutations in GRIN3B have been implicated in various neurological disorders, including schizophrenia, Huntington's disease, and rare forms of epilepsy, highlighting its importance as a potential therapeutic target for modulating glutamatergic signaling without completely abolishing NMDAR function.

Picture

Western Blot

WB result of GluN3B Recombinant Rabbit mAb
Primary antibody: GluN3B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: CHO transfected with empty vector whole cell lysate 20 µg
Lane 2: CHO transfected with GluN3B-Myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 113 kDa
Observed MW: 115 kDa

Immunocytochemistry

ICC shows positive staining in GluN3B-Myc-His fusion protein transfected CHO cells (top panel) and negative staining in vector-transfected CHO cells (below panel). Anti- GluN3B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).