Flow cytometric analysis of mouse MERTK expression on BALB/c mouse peritoneal exudates cells. BALB/c mouse peritoneal exudates cells were stained with Brilliant Violet 421™ Rat Anti-Mouse F4/80 Antibody and either Biotin Rat IgG2a, κ Isotype Control (left panel) or SDT Biotin Rat Anti-Mouse MERTK Antibody (right panel) at 2μl/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse MERTK Antibody (S-R603)
Biotin Rat Anti-Mouse MERTK Antibody (S-R603)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | MERTK |
| Synonyms | Tyrosine-protein kinase Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; Mer; Mertk |
| Location | Cell membrane |
| Accession | Q60805 |
| Clone Number | S-R603 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse peritoneal exudates cells |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
MERTK, also known as MER proto-oncogene tyrosine kinase, is a type I receptor tyrosine kinase and a member of the TAM (TYRO3, AXL, and MERTK) family. It plays crucial roles in various physiological processes, including cell survival, migration, differentiation, and the phagocytosis of apoptotic cells. MERTK is activated by binding to ligands such as GAS6 and PROS1, which triggers autophosphorylation and downstream signaling through pathways like PI3K/AKT, MAPK, and STAT. This receptor is highly expressed in immune cells like macrophages and dendritic cells, and its activation promotes an anti-inflammatory M2 phenotype in macrophages. In the retina, MERTK is essential for the phagocytosis of rod outer segment fragments by the retinal pigment epithelium. Abnormal expression of MERTK has been linked to several diseases, including retinitis pigmentosa and various cancers, making it a potential therapeutic target.
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