1 1

Biotin Mouse Anti-Mouse H-2Kd Antibody (S-R647)

Biotin Mouse Anti-Mouse H-2Kd Antibody (S-R647)

Catalog Number: S0B8881 Application: FCM Reactivity: Mouse Conjugation: Biotin Brand: Starter
Share:
Price:
$85 USD
$85 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Host Mouse
Antigen H-2Kd
Synonyms H-2 class I histocompatibility antigen, K-D alpha chain; H-2K(D); H2-K1; H2-K
Location Membrane
Accession P01902
Clone Number S-R647
Antibody Type Mouse mAb
Isotype IgG2a,k
Application FCM
Reactivity Ms
Positive Sample BALB/c mouse splenocytes
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Biotin
Physical Appearance Liquid
Storage Buffer

PBS pH7.4, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1μg per million cells in 100μl volume Ms

Background

H-2Kd protein is a major histocompatibility complex (MHC) class I molecule expressed in mice, particularly in BALB/c strains. It plays a critical role in antigen presentation by binding to antigenic peptides derived from pathogens, such as influenza nucleoprotein, and presenting them to CD8+ T cells to initiate an immune response. The crystal structure of H-2Kd in complex with beta-2 microglobulin and an influenza-derived peptide has been elucidated, revealing specific binding motifs and the structural basis for peptide recognition. This understanding is crucial for studies on immune responses and vaccine development.

Picture

FC

Flow cytometric analysis of mouse H-2Kd expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Biotin Mouse IgG2a, κ Isotype Control (black line histogram) or SDT Biotin Mouse Anti-Mouse H-2Kd Antibody (red line histogram) at 2 μl/test followed by Sav-iFluor 488, cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.