WB result of Myc tag Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: Myc tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: 293T transfected with H3.3-Myc-His tag whole cell Lysate 5 µg
Predicted MW: 19 kDa
Observed MW: 19 kDa
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Immunogen | Synthetic Peptide |
| Clone Number | S-114-13 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | null |
Background
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Picture
Picture
Western Blot
