WB result of His tag Recombinant Rabbit mAb (HRP Conjugate)
Lysate: Angiopoietin-1 (277-498) His Tag Protein, Mouse 20 ng
Lane 1: His tag Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: His tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: His tag Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: His tag Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 28~33 kDa
Observed MW: 28 kDa
His tag (C-terminal) Recombinant Rabbit mAb (HRP Conjugate) (S-1398-10)
His tag (C-terminal) Recombinant Rabbit mAb (HRP Conjugate) (S-1398-10)
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$95 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | His tag |
| Immunogen | Synthetic Peptide |
| Clone Number | S-1398-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 |
Background
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.
Picture
Picture
Western Blot
