WB result of GST Tag Rabbit mAb (HRP Conjugate)
Lysate: Recombinant Schistosoma Japonicum GST Protein 20ng
Primary antibody:
Lane 1: GST Rabbit mAb at 1/5000 dilution
Lane 2: GST Rabbit mAb at 1/10000 dilution
Lane 3: GST Rabbit mAb at 1/20000 dilution
Predicted MW: 25 kDa
Observed MW: 24 kDa
GST Tag Recombinant Rabbit mAb (HRP Conjugate) (S-372-19)
GST Tag Recombinant Rabbit mAb (HRP Conjugate) (S-372-19)
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$80 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST |
| Immunogen | Recombinant Protein |
| Accession | P08515 |
| Clone Number | S-372-19 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:20000 | null |
Background
Glutathione S-transferases (GSTs), previously known as ligandins, are a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification [PMID: 15717864]. A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 kDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein.
Picture
Picture
Western Blot
