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FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) (S-589-8)

FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) (S-589-8)

Catalog Number: S0B1648 Application: WB Reactivity: Other Conjugation: HRP Brand: Starter
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Regular price $80 USD
Regular price Sale price $80 USD
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Product Details

Product Specification


Host Rabbit
Antigen FLAG Tag
Immunogen Synthetic Peptide
Clone Number S-589-8
Antibody Type Recombinant mAb
Isotype IgG
Application WB
Reactivity Species Independent
Purification Protein A
Concentration 2 mg/ml
Conjugation HRP
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied.

Dilution


application dilution species
WB 1:5000-1:10000 Species Independent

Background

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.

Picture

Western Blot

WB result of FLAG Tag Recombinant Rabbit mAb (HRP Conjugate)
Lysate: 293T transfected with Histone H3-Flag-V5 whole cell lysate 20 µg
Primary antibody:
Lane 1: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa

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