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FLAG Tag Recombinant Rabbit mAb (FITC Conjugate) (S-589-8)

FLAG Tag Recombinant Rabbit mAb (FITC Conjugate) (S-589-8)

Catalog Number: S0B1840 Application: FCM,ICC Reactivity: Other Conjugation: FITC Brand: Starter
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Regular price $180 USD
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Product Details

Product Specification


Host Rabbit
Antigen FLAG Tag
Immunogen Synthetic Peptide
Clone Number S-589-8
Antibody Type Recombinant mAb
Isotype IgG
Application ICC, ICFCM
Reactivity Species Independent
Positive Sample H3-Flag-V5 transfected 293T cells
Purification Protein A
Concentration 2 mg/ml
Conjugation FITC
Physical Appearance Liquid
Storage Buffer

PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Dilution


application dilution species
ICC 1:500 Species Independent
ICFCM 1:800 Species Independent

Background

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.

Picture

FC

Flag Tag transfected 293T (Human embryonic kidney epithelial cell, Right panel) or 293T (Left panel) was fixed with 4% PFA and permeabilized 90% methanol ,then cells were stained with either FITC Rabbit IgG Isotype Control (Black line histogram) or SDT FLAG Tag Recombinant Rabbit mAb (FITC Conjugate) (S-589-8) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

Immunocytochemistry

ICC shows positive staining in Histone H3-Flag-V5 transfected 293T cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti- FLAG Tag (FITC Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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