Measured by its ability to inhibit BMP-2-induced luciferase reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is 64-77ng/mL in the presence of 100ng/mL Recombinant Human BMP-2.
Noggin Protein, Mouse
Noggin Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Noggin |
| Accession | P97466 |
| Amino Acid Sequence | Gln28-Cys232 |
| Expression System | CHO |
| Molecular Weight | 29-32 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Nature. 2000 Feb 10;403(6770):658-61. |
Background
Mouse Noggin is a 64 kDa secreted homodimeric glycoprotein that functions as a potent antagonist of Bone Morphogenetic Protein (BMP) signaling. Encoded by the Nog gene, Noggin directly binds BMP ligands (BMP-2, -4, -5, -7) and Growth Differentiation Factors (GDF-5, -6) with high affinity, preventing their interaction with cognate receptors and subsequent downstream Smad-dependent signaling cascade activation. The protein is expressed in key developmental organizers including the node, notochord, dorsal somite, and roof plate, where it establishes gradients of BMP activity essential for patterning.
In the adult mouse brain, Noggin is expressed in the subventricular zone (SVZ) where it creates a neurogenic niche by antagonizing endogenous BMPs. This promotes neuronal differentiation of neural stem cells while suppressing astrocytic fate, thereby maintaining neurogenesis throughout adulthood.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
