The PARP1 activity was assayed with ELISA. The reaction was performed by: 1). Histone protein was coated on a 96-well plate. 2).A biotinylated NAD+ mix is incubated with varying different concentration of the PARP1 enzyme and an activated DNA. 3).The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
PARP1, His Tag Protein
PARP1, His Tag Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1, PARP-1 |
| Accession | P09874 |
| Amino Acid Sequence | A2-W1014(end) |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 115077.0 |
| Purity | >85% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Hepes, 300 mM NaCl, 1mM DTT, pH7.0 |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg PARP1
2 3 μg PARP1
