The IKZF3 activity was detected by HTRF binding assay. The reaction was performed by incubating the IKZF3 protein, CRBN and CC-885 at 25℃ for 30 min, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Human IKZF3 Protein
Human IKZF3 Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | ZNFN1A3 |
| Accession | Q9UKT9 |
| Amino Acid Sequence |
HTGERPFQCNQCGASFTQKGNLLRHIKLHT |
| Expression System | E.coli |
| Purity |
＞95%, by SDS-PAGE under reducing conditions ＞95%，as determined by HPLC |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
| Stability & Storage |
Stable for
12 months upon stored at -80℃ from the date of receipt. And avoid repeated
freeze-thaws cycles.
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Picture
Picture
Bioactivity
SDS-PAGE
SEC-HPLC
