CD155/PVR Fc Chimera Protein, Human

Catalog Number: UA010118 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$637.00 SGD

Size: 100μg 500μg

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Product Details

Product Specification

Species	Human
Accession	P15151
Amino Acid Sequence	Trp21-Asn343, with C-terminal Human IgG1 Fc WPPPGTGDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHGESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGNYTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTGGRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVTCKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARSNPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGARQAELTVQVKEGPPSEHSGISRNGGGSGGGSPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Expression System	HEK293
Molecular Weight	79-95kDa (Reducing)
Purity	>95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	Human Fc Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.

Background

CD155 is a member of the immunoglobulin superfamily also known as the human receptor for poliovirus (PVR). The full length (or PVR alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20aa signal sequence, a 323aa extracellular region, a 24aa TM segment and a 50aa cytoplasmic tail. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhesion, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity. CD155 is expressed at high levels in several human malignancies and seems to have pro tumorigenic and therapeutically attractive properties that are currently being investigated in the field of recombinant oncolytic vitro therapy. More intriguingly, PVR participates in a considerable number of immunoregulatory functions through its interactions with activating and inhibitory immune cell receptors. These functions are often modified in the tumor microenvironment, contributing to tumor immunosuppression.

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Bioactivity

Immobilized DNAM-1/CD226 His Tag, Human (Cat. No. UA010072) at 2μg/mL (100μL/well) can bind CD155/PVR Fc Chimera, Human (Cat. No. UA010118) with EC50 of 0.317μg/ml.

Immobilized TIGIT His Tag, Cynomolgus (Cat. No. UA010106) at 2.5μg/mL (100μL/well) can bind CD155/PVR Fc Chimera, Human (Cat. No. UA010118) with EC50 of 1.018μg/ml.

Immobilized TIGIT His Tag, Human (Cat. No. UA010136) at 2.5μg/ml (100μl/well) can bind CD155/PVR Fc Chimera, Human (Cat. No. UA010118) with EC50 of 0.234μg/ml.

Anti-His antibody Immobilized on CM5 Chip captured TIGIT His Tag, Human (Cat. No. UA010136), can bind CD155/PVR Fc Chimera, Human (Cat. No. UA010118) with an affinity constant of 0.153μM as determined in SPR assay.

Anti-His antibody Immobilized on CM5 Chip captured DNAM-1/CD226 His Tag, Human (Cat. No. UA010072), can bind CD155/PVR Fc Chimera, Human (Cat. No. UA010118) with an affinity constant of 0.419μM as determined in SPR assay.

Anti-His antibody Immobilized on CM5 Chip captured TIGIT His Tag, Cynomolgus (Cat. No. UA010106), can bind CD155/PVR Fc Chimera, Human (Cat. No. UA010118) with an affinity constant of 0.124μM as determined in SPR assay.