WB result of FOXL2 Recombinant Rabbit mAb
Primary antibody: FOXL2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 50 kDa
This blot was developed with high sensitivity substrate
FOXL2 Recombinant Rabbit mAb (SDT-557-361)
FOXL2 Recombinant Rabbit mAb (SDT-557-361)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FOXL2 |
| Synonyms | Forkhead box protein L2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P58012 |
| Clone Number | SDT-557-361 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | K562, A549, mouse ovary |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms |
| IHC-P | 1:250 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| IF | 1:500 | Ms |
Background
FOXL2 is a winged-helix/forkhead transcription factor encoded on chromosome 3q23 that is indispensable for female sex determination, ovarian somatic-cell differentiation, granulosa-cell function, and eyelid morphogenesis; mutations or deletions in FOXL2 cause blepharophimosis–ptosis–epicanthus inversus syndrome and are pathognomonically associated with adult granulosa-cell tumours, while somatic missense mutations (most prominently C134W) constitutively activate the protein, driving oncogenesis through altered transcriptional programs that maintain granulosa-cell lineage identity and suppress apoptosis, thereby highlighting its dual role as a gatekeeper of ovarian development and a lineage-specific tumour suppressor/oncogene.
Picture
Picture
Western Blot
WB result of FOXL2 Recombinant Rabbit mAb
Primary antibody: FOXL2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse ovary lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 50 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human ovary. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian granulosa cell tumor. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse ovary. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat ovary. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A549 cells (top panel) and negative staining in MCF7 cells (below panel). Anti-FOXL2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse ovary. Anti-FOXL2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
