mIHC shows positive staining in paraffin-embedded human squamous cell carcinoma. Anti- CK-Pan Rabbit mAb (S0B2118) was used at 1/200 dilution (Green), Anti-CD45 Rabbit mAb (S0B2087) was used at 1/40 dilution (Yellow), and Anti-CD31 Rabbit mAb (S0B2009) was used at 1/100 dilution (Red), followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with mIHC staining protocol.
Product Details
Product Details
Product Specification
Antibody Type | Recombinant mAb |
Application | mIHC |
Reactivity | Hu |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
application | dilution | species |
mIHC | 1:50-1:200 |
Background
CK-Pan, CD45, CD31 Antibody Panel contains 4 recombinant monoclonal antibodies against CK-Pan, CD45, CD31. They are provided as a sampler panel.
Panel contains:
- S-RMab® CK-Pan Recombinant Rabbit mAb (SDT-P001) S0B2118
- S-RMab® CD45 Recombinant Rabbit mAb (SDT-R035) S0B2087
- CD31 Recombinant Rabbit mAb (SDT-008-17) S0B2009
Picture
Picture
Contents & Protocol
Multiple Immunohistochemistry
