WB result of CD25/IL-2Rα Recombinant Rabbit mAb
Primary antibody: CD25/IL-2Rα Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: CTLL-2 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate; C2C12 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 38-70 kDa
This blot was developed with high sensitivity substrate
CD25/IL-2Rα Recombinant Rabbit mAb (SDT-2234-65)
CD25/IL-2Rα Recombinant Rabbit mAb (SDT-2234-65)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD25 |
| Synonyms | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; Il2r; Il2ra |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | P01590 |
| Clone Number | SDT-2234-65 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Ms |
| Positive Sample | CTLL-2 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:100-1:200 | Ms |
| ICC | 1:500 | Ms |
Background
CD25, also known as the interleukin-2 receptor alpha chain (IL2RA), is a type I transmembrane glycoprotein that plays a crucial role in the immune system. It is primarily expressed on the surface of activated T cells and regulatory T cells (Tregs). CD25 is a key component of the high-affinity IL-2 receptor complex, which also includes the IL-2 receptor beta and gamma chains. By itself, CD25 can bind IL-2 with low affinity, but when combined with the beta and gamma chains, it forms a high-affinity receptor that initiates intracellular signaling pathways such as MAPK and JAK/STAT. This receptor complex is essential for the proliferation and activation of T cells, as well as the regulation of immune tolerance by controlling the activity of Tregs. Mutations in the IL2RA gene can lead to deficiencies in IL-2 receptor function, highlighting its importance in maintaining immune homeostasis.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse thymus. Anti-CD25/IL-2Rα antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD25/IL-2Rα antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-CD25/IL-2Rα antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-CD25/IL-2Rα antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in CTLL-2 cells (top panel) and negative staining in C2C12 cells (below panel). Anti- CD25/IL-2Rα antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
