WB result of Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb
Primary antibody: Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 180 kDa
Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb, PBS Only (SDT-2662-122)
Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb, PBS Only (SDT-2662-122)
Price:
Regular price
$131.00 SGD
Regular price
Sale price
$131.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Angiotensin Converting Enzyme 1 |
| Synonyms | Angiotensin-converting enzyme; ACE; Dipeptidyl carboxypeptidase I; Kininase II; CD143; DCP; DCP1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Secreted, Cell membrane |
| Accession | P12821 |
| Clone Number | SDT-2662-122 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse kidney, mouse lung, rat lung |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
Angiotensin-Converting Enzyme 1 (ACE1) is a zinc-dependent dipeptidyl carboxypeptidase expressed as a 1,306-amino-acid type-I transmembrane glycoprotein, with the bulk of its structure (residues 1–1230) projecting into the extracellular space and containing two homologous catalytic N- and C-terminal domains, each bearing the characteristic HEXXH zinc-binding motif; by cleaving the decapeptide angiotensin I into the potent vasoconstrictor octapeptide angiotensin II, degrading the vasodilator bradykinin, and modulating additional vasoactive peptides, ACE1 sits at the apex of the renin–angiotensin–aldosterone system, thereby governing systemic blood pressure, extracellular fluid volume, and cardiovascular remodeling, while its genetic polymorphisms—most notably the Alu insertion/deletion (I/D) variant in intron 16—have been linked to inter-individual differences in circulating enzyme levels and risk for hypertension, myocardial infarction, diabetic nephropathy, and COVID-19 severity, making ACE1 a cornerstone target for therapeutic inhibitors such as captopril, enalapril, and lisinopril that are now mainstays in treating hypertension and heart failure.
Picture
Picture
Western Blot
WB result of Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb
Primary antibody: Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 180 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
