Schematic diagram of Mouse MDSCs Panel Kit (CD11b/LY6C/LY6G/Gr-1)
Mouse MDSCs Panel Kit (CD11b/LY6C/LY6G/Gr-1)
Mouse MDSCs Panel Kit (CD11b/LY6C/LY6G/Gr-1)
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Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | Mouse bone marrow, Mouse splenocytes |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This four‑color panel is designed for detecting myeloid‑derived suppressor cell (MDSC) subsets in mouse tissue cells. CD11b was included as a general myeloid cell marker, and Gr‑1 was included to identify the total myeloid population. Finally, the expression pattern of Ly6C and Ly6G on the gated myeloid cells can distinguish monocytic MDSCs (M‑MDSC) and granulocytic MDSCs (G‑MDSC). The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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Picture
FC
Mouse bone marrow were stained using the Mouse MDSCs Panel Kit consisting of anti-mouse CD11b FITC, anti-mouse Ly6C PE, anti-mouse Ly6G APC, and anti-mouse Ly-6G/Ly-6C (Gr-1) Pacific Blue conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the cells excluding cellular debris.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD11b FITC-A vs Ly-6G/Ly-6C (Gr-1) Pacific Blue-A dot plot show the myeloid cells with the live cells gate applied.
4. Create a Ly6G APC-A vs Ly6C PE-A dot plot with the myeloid cells gate applied. Create gates to encompass Ly6G-Ly6Chi cells (M-MDSC), Ly6G+Ly6Gmid cells (G-MDSC)
Mouse splenocytes were stained using the Mouse MDSCs Panel Kit consisting of anti-mouse CD11b FITC, anti-mouse Ly6C PE, anti-mouse Ly6G APC, and anti-mouse Ly-6G/Ly-6C (Gr-1) Pacific Blue conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the cells excluding cellular debris.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD11b FITC-A vs Ly-6G/Ly-6C (Gr-1) Pacific Blue-A dot plot show the myeloid cells with the live cells gate applied.
4. Create a Ly6G APC-A vs Ly6C PE-A dot plot with the myeloid cells gate applied. Create gates to encompass Ly6G-Ly6Chi cells (M-MDSC), Ly6G+Ly6Gmid cells (G-MDSC)
