Siglec-8 mFc Chimera Protein, Human
Siglec-8 mFc Chimera Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Siglec8, Siglec-8, SAF2, SAF2SAF-2, SAF-2, CD329 antigen, CDw329 |
| Accession | Q9NYZ4 |
| Amino Acid Sequence | Met17-Ala 363, with C-terminal Mouse IgG2a Fc |
| Expression System | HEK293 |
| Molecular Weight | 75-80 kDa(Reducing) |
| Purity | ＞95%, by SDS-PAGE under reducing conditions |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Mouse Fc Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS pH7.2, 5% trehalose |
| Reconstitution | Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation . |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules . Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 115 aa cytoplasmic domain with two tyrosine based signaling motifs . Alternative splicing generates additional isoforms that either lack most of the second Ig-like domain or have a substituted cytoplasmic domain without the signaling motifs . Siglec-8 is expressed on eosinophils, basophils, and mast cells , and it shows a binding preference for the carbohydrate 6-O sulfated sLex . In mouse, Siglec-F is a functional paralog of human Siglec-8, as shown by its similar expression profile and ligand specificity . Cross-linking of Siglec-8 inhibits Fc epsilon RI alpha induced mast cell degranulation . It also induces eosinophil apoptosis, an effect which is enhanced by the eosinophil-activating cytokines IL-5, IL-33, and GM-CSF .
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