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PLK4 His&Flag Tag Protein, Human

PLK4 His&Flag Tag Protein, Human

Catalog Number: UA080505 Brand: UA BIOSCIENCE
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Regular price $940.00 SGD
Regular price Sale price $940.00 SGD
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Product Details

Product Specification


Species Human
Synonyms SAK,STK18
Accession O00444
Amino Acid Sequence

A2-H970(end) ; N-terminal GST tag-TEV, C-terminal Flag tag

Expression System Baculovirus-InsectCells
Molecular Weight 136.6 kDa
Purity >70% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag, Flag Tag
Physical Appearance Liquid
Storage Buffer

50 mM Tris, 150 mM NaCl, 5 mM GSH, 1 mM DTT, 0.05% Brij35,10% glycerol, pH7.5

Stability & Storage

Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.

Picture

Bioactivity

The PLK4 kinase activity was quantified using the ADP-Glo assay. The reaction was performed by incubating the PLK4 protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.

SDS-PAGE