Nectin-1/PVRL1/CD111 His Tag Protein, Human
Nectin-1/PVRL1/CD111 His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Poliovirus Receptor-Related Protein 1; Herpes Virus Entry Mediator C; Herpesvirus Entry Mediator C; HveC; Herpesvirus Ig-Like Receptor; HIgR; Nectin-1; CD111; PVRL1; HVEC; PRR1 |
| Accession | Q15223-1 |
| Amino Acid Sequence | Gln31-Thr334, with C-terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | 45-55kDa(Reducing) |
| Purity | ＞95%, by SDS-PAGE under reducing conditions |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation . |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
Nectin-1, also referred to as ED4, is a poliovirus receptor- related 1 protein is a part of the Nectin family. Nectin-1 endorses cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions among PVRL1/nectin-1 & PVRL4/nectin-4 and among PVRL1/nectin-1 & PVRL3/nectin-3 have been found. Nectin-1 acts as an entry receptor for herpes simplex virus & pseudorabies virus as well. Likewise, Neurite outgrowth-promoting activity has been shown by Nectin-1.
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Bioactivity
Anti-His antibody Immobilized on CM5 Chip captured Nectin-1 His Tag, Human (Cat. No. UA010017), can bind Nectin-3 Fc Chimera, Human(Cat. No. UA010019) with an affinity constant of 17.31 nM as determined in SPR assay.
SDS-PAGE
Nectin-1/PVRL1/CD111 His Tag, Human，1μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%.
