1μg (R: reducing condition, N: non-reducing condition).
NCAM-1/CD56 (ΔQ609, G610R, M621R) His Tag Protein, Human
NCAM-1/CD56 (ΔQ609, G610R, M621R) His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Neural cell adhesion molecule 1,CD56,NCAM1,NCAM,Neural Cell Adhesion Molecule,NCAM,CD56 Antigen,N-CAM-1,MSK39,NCAM-1,Antigen Recognized By Monoclonal Antibody 5.1H11 |
| Accession | P13591-2 |
| Amino Acid Sequence | Leu20-Gly718 (ΔQ609, Gly610Arg, Met621Arg) with His Tag at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 90-110kDa (Reducing) |
| Purity | >95% as determined by SDS-PAGE & SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Guven A. et al. (2021) Development and validation of an immunoassay for quantification of NCAM-1 in human plasma. Journal of Pharmaceutical and Biomedical Analysis. 197: 113981. |
Background
Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on neurons, glia, skeletal muscle, and natural killer cells. It plays a key role in cell-cell adhesion and is crucial for nervous system development. Additionally, NCAM-1 is involved in the expansion of T cells and dendritic cells, which are vital for immune surveillance.
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SDS-PAGE
SEC-HPLC
The purity of NCAM-1/CD56 (ΔQ609, G610R, M621R) His Tag Protein, Human is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized NCAM-1/CD56 (ΔQ609, G610R, M621R) His Tag Protein, Human (Cat. No. UA011224) at 1.0μg/mL (100μL/well) can bind Anti-CD56 Reference Antibody (Lorvotuzumab) (Cat. No. UA010990) with EC50 of 1.67-2.42ng/mL.
