The Kras[G12C](GDP Loaded) activity was detected using HTRF technology. The reaction was performed by incubating the Kras[G12C](GDP Loaded) protein, GTP and SOS1 at 25℃ for 60 min, then adding cRAF at 25℃ for 15 min and adding the beads at 25℃ for 60 min, reading ratio 665/620 with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | GTPase KRas,KRAS2,RASK2 |
Accession | P01116-2 |
Amino Acid Sequence | M1-K169,with N-terminal 8x His tag,C-terminal Avi |
Expression System | E.coli |
Molecular Weight | 23.6 kDa |
Purity | >85% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | Avi Tag, His Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
 His&Avi Tag Protein, Human](http://www.antbioinc.com/cdn/shop/files/ua_55d99cb7-1600-449a-9919-107c251a89ae.png?v=1757556136&width=1445)