2μg (R: reducing condition, N: non-reducing condition).
EDNRB EGFP-Flag-His Tag Protein, Human(nine mutants, Detergent)
EDNRB EGFP-Flag-His Tag Protein, Human(nine mutants, Detergent)
Price:
Regular price
$1,400 USD
Regular price
Sale price
$1,400 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Endothelin receptor type B, EDNRB, ABCDS, HSCR, HSCR2, ET-B, ET-BR, ETB1, ETBR, ETRB, WS4A |
| Accession | P24530 |
| Concentration | 1.13 mg/ml by Nanodrop |
| Amino Acid Sequence | EDNRB protein with egfp-Flag-His tag, nine mutants and tev site |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 75-90 kDa (Reducing) |
| Purity | ＞90% by SDS-PAGE &＞90% by SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tags & Cleavage sites | tev site |
| Tag | His Tag, Flag Tag |
| Physical Appearance | Liquid with detergent |
| Storage Buffer | 10 mM Hepes, pH 7.5, 200 mM NaCl, 0.01 % LMNG, 0.001 % CHS. |
| Stability & Storage | ·6 months from date of receipt, -60 to -80 °C as supplied. |
| Reference | 1. Ji, Yujie, et al. "Structural basis of peptide recognition and activation of endothelin receptors." Nature Communications 14.1 (2023): 1268. |
Background
Endothelin Receptor B (EDNRB) is a G protein-coupled receptor (GPCR) whose activity and function are regulated by G proteins. These G proteins activate a phosphatidylinositol-calcium second messenger system. EDNRB is widely expressed throughout the human body and plays a crucial role in regulating various physiological activities.
EDNRB binds to endothelin (ET) ligands and transmits signals into the cell via an intracellular Ca²⁺/phospholipid-dependent protein kinase second messenger system. This process promotes the rapid release of calcium from the endoplasmic reticulum and sarcoplasmic reticulum, facilitating downstream signaling events.
Endothelin exerts its biological functions through its receptors, such as EDNRB. Together, endothelin and its receptors (ETRs) form the endothelin axis. The endothelin axis has been implicated in various physiological and pathological processes. In the context of oncology, the endothelin axis exhibits pro-tumor effects, promoting tumor growth, metastasis, anti-apoptosis, and angiogenesis.
Picture
Picture
SDS-PAGE
SEC-HPLC
The purity of EDNRB protein egfp-Flag-His tag (nine mutants, detergent) Protein his tag thrombin site in detergent is greater than 90% as determined by SEC-HPLC.
ELISA
Immobilized EDNRB Rabbit pAb (A2908) at 0.5μg/mL (100μL/well) can bind EDNRB protein egfp-Flag-His tag (nine mutants, detergent), Human with EC50 of 41.30-67.52ng/mL.
