The CRBN/DDB1 activity was detected using TR-FRET technology. The reaction was performed by incubating the CRBN/DDB1 protein and Thalidomide-BODIPY, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 520/490 with BMG.
CRBN/DDB1 Protein, Dog
CRBN/DDB1 Protein, Dog
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Product Details
Product Details
Product Specification
| Accession | A0A8C0RYL7、A0A8C0QFD6 |
| Amino Acid Sequence | CRBN: M1-L444(end); N-terminal 8x His tagDDB1: M1-H1140(end); No tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | CRBN: 53.4 kDa; DDB1: 127.0 kDa |
| Purity | >90% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Background
Recombinant dog CRBN with N-terminal 8x His tag was co-expressed with recombinant dog DDB1 protein. Recombinant dog CRBN/DDB1 protein with N-terminal 8x His tag was purified by Ni-NTA affinity and followed by desalting. The CRBN/DDB1 protein showed high activity in TR-FRET assay.
Picture
Picture
Bioactivity
SDS-PAGE
