| Species | Human |
| Synonyms | Centromere-associated protein E,CENP-E |
| Accession | Q02224 |
| Amino Acid Sequence | M1-S339,MAEEGAVAVCVRVRPLNSREESLGETAQVYWKTDNNVIYQVDGSKSFNFDRVFHGNETTKNVYEEIAAPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSEDHLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCGTQKMKPLIIREDVNRNVYVADLTEEVVYTSEMALKWITKGEKSRHYGETKMNQRSSRSHTIFRMILESREKGEPSNCEGSVKVSHLNLVDLAGSERAAQTGAAGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTITPVSFDETLTALQFASTAKYMKNTPYVNEVS |
| Expression System | E.coli |
| Molecular Weight | 65.2 kDa |
| Purity | >40% by SDS-PAGE |
| Endotoxin | <2EU/μg |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5 |
| Stability & Storage |
CENP-E(Motor Domain) activity test
The CENP-E(Motor Domain) activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CENP-E(Motor Domain) protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU with BMG.
