CD47 Fc Chimera Protein, Human

Catalog Number: UA010006 Brand: UA BIOSCIENCE

Price:

$260 USD

Size: 25μg 100μg

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Product Details

Product Specification

Species	Human
Synonyms	CD47 Fc Chimera protein ;antigen identified by monoclonal 1D8, Antigenic surface determinant protein OA3, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 antigen, CD47 glycoprotein, CD47 molecule, CD47, IAP, IAPintegrin associated protein, Integrin-associated protein, leukocyte surface antigen CD47, MER6, MER6integrin-associated signal transducer, OA3, Protein MER6, Rh-related antigen
Accession	Q08722
Amino Acid Sequence	Gln19-Pro139, with C-terminal Human IgG Fc
Expression System	HEK293
Molecular Weight	55~65 kDa(Reducing)
Purity	＞95%, by SDS-PAGE under reducing conditions
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	Human Fc Tag
Physical Appearance	Lyophilized powder
Storage Buffer	PBS, pH7.2, 5% trehalose
Reconstitution	Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.

Background

CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.

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Bioactivity

Immobilized Human SIRP-α His tag (Cat. No. UA010009) at 1μg/mL (100μL/well) can bind CD47 Fc Chimera, Human(Cat. No. UA010006) with EC50 of 3.91-5.60ng/mL.

Immobilized SIRP-α/CD172a His Tag, Cynomolgus (Cat. No. UA010772) at 2.0μg/mL (100μL/well) can bind CD47 Fc Chimera, Human (Cat. No. UA010006) with EC50 of 1.34-1.91 ng/mL.

SDS-PAGE

CD47 Fc Chimera, Human, 2μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.

SPR

Anti-His antibody Immobilized on CM5 Chip captured SIRP-α His Tag, Human (Cat. No. UA010009), can bind CD47 Fc Chimera, Human(Cat. No. UA010006) with an affinity constant of 4.828 nM as determined in SPR assay.

Protein A Chip captured CD47 Fc Chimera, Human (Cat. No. UA010006), can bind SIRP-α/CD172a His Tag, Cynomolgus(Cat. No. UA010772) with an affinity constant of 43.88nM as determined in SPR assay.