2e5 of transient transfected Rituximab-CD20-scFV CAR-293 cells were stained with 0.1ug CD20 Flag&His Tag Protein, Human, (Cat. No. UA060018) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity. 2e5 of transient transfected Rituximab-CD20-scFV CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.
CD20 Flag&His Tag Protein, Human (Detergent)
CD20 Flag&His Tag Protein, Human (Detergent)
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | B-lymphocyte surface antigen B1，MS4A1，B-lymphocyte antigen CD20，Membrane Spanning 4-Domains A1，Leukocyte Surface Antigen Leu-16，CD20 Antigen，CD20，Bp35，Membrane-Spanning 4-Domains Subfamily A Member 1，CD20 Receptor，LEU-16，CVID5，MS4A2，B1，S7，Antigens， CD20，Membrane-Spanning 4-Domains， Subfamily A， Member 1，B-Lymphocyte Cell-Surface Antigen B1 |
| Accession | P11836 |
| Concentration | 0.576mg/ml by Nanodrop |
| Amino Acid Sequence | Full length, with Flag tag at N-Terminal, and His tag at C-Terminal |
| Expression System | HEK293 |
| Molecular Weight | 35.4 kDa |
| Purity | ＞90% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tags & Cleavage sites | N/A |
| Tag | His Tag, Flag Tag |
| Physical Appearance | Liquid with detergent |
| Storage Buffer | 25 mM Tris, pH 7.5, 150 mM NaCl, 0. 02 % DDM. |
| Stability & Storage | ·6 months from date of receipt, -60 to -80 °C as supplied. |
| Reference | 1. Rougé L, Chiang N, Steffek M, et al. Structure of CD20 in complex with the therapeutic monoclonal antibody rituximab[J]. Science, 2020, 367(6483): 1224-1230. |
Background
CD20 is a 33-37 kDa non-glycosylated protein present on the surface of normal and malignant B lymphocytes and is a member of the MS4A (membrane-spanning 4-domain family A) protein family. The CD20 protein comprises four hydrophobic transmembrane domains, one intracellular domain, and two extracellular domains (a large loop and a small loop), with both the N- and C-termini located within the cytosol. CD20 is associated with several key molecules, including the major histocompatibility complex class II (MHCII), CD40 molecule, B-cell receptor (BCR), and the C-terminal src kinase-binding protein (CBP). CBP interacts with Src kinases such as LYN, FYN, and LCK.
A deficiency in CD20 has been shown to result in a reduced number of circulating memory B cells, decreased isotype switching of immunoglobulins (Ig), and lower IgG antibody levels. It is also linked to various pathophysiological diseases and disorders.
CD20 plays a crucial role in multiple physiological processes and is thus a promising therapeutic target. The CD20 protein product has the potential to enhance drug discovery efforts in related fields.
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SDS-PAGE
2 μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized CD20 Flag&His tag, Human (Cat. No. UA060018) at 5μg/mL (100μL/well) can bind S-RMab® CD20 Recombinant Rabbit mAb (SDT-R133) (Cat. No. S0B2158) with EC50 of 0.30-0.43ng/mL.
