1μg (N: non-reducing condition).
Biotinylated CD27 Ligand/CD70 Fc&His&Avi Trimer design Protein, Human
Biotinylated CD27 Ligand/CD70 Fc&His&Avi Trimer design Protein, Human
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$560 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CD27 Ligand; CD27-L; CD27L; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7 |
| Accession | P32970 |
| Amino Acid Sequence | Gly54-Pro193 with Human IgG1 Fc & His Tag & Avi Tag at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 180-250kDa (Non-Reducing) |
| Purity | >95% by SDS-PAGE &SEC-HPLC |
| Endotoxin | <1EU/μg |
| Conjugation | Biotin |
| Tag | Avi Tag, His Tag, Human IgG1 Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4,5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Goodwin RG, Alderson MR, Smith CA, et al. Molecular and biological characterization of a ligand for CD27 defines a new family of cytokines with homology to tumor necrosis factor. Cell. 1993;73:447-456. |
Background
The TNFSF7 gene (Tumor Necrosis Factor Ligand Superfamily, member 7), located on chromosome 19p13, encodes a surface antigen expressed on activated T and B lymphocytes but not on resting cells. TNFSF7 is a 19 kDa protein comprising a 20-amino acid hydrophilic N-terminal domain without a signal sequence, an 18-amino acid hydrophobic transmembrane anchor region, and a C-terminal domain with two potential N-linked glycosylation sites. This structure classifies TNFSF7 as a type II transmembrane protein.
TNFSF7 is expressed on a subset of B, T, and NK cells, where it plays a costimulatory role in immune cell activation. It shares homology with other TNF receptor family ligands, including TNF-alpha, TNF-beta, and the CD40 ligand, with 19 to 24% amino acid sequence identity in the extracellular region.
In the context of breast cancer, TNFSF7 gene expression is epigenetically downregulated through DNA hypermethylation in its promoter region, as observed in the isogenic MCF10 model during cancer progression.
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SDS-PAGE
SEC-HPLC
The purity of Biotinylated CD27 Ligand/CD70 Fc&His&Avi Trimer design Protein, Human is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized CD27/TNFRSF7 His Tag Protein, Human (Cat. No. UA010124) at 2.0μg/mL (100μL/well) can bind Biotinylated CD27 Ligand/CD70 Fc&His&Avi Trimer design Protein, Human (Cat. No. UA011164) with EC50 of 12.92-15.78ng/mL.
