The Biotin-KRAS[G12C] activity was quantified using a HTRF-based assay. The reaction was performed by incubating the Biotin-KRAS[G12C] protein and substrate, the donor and receptor at 25℃ for 60 min, then measuring the 665nm/615nm fluorescence signal ratio on BMG.
Biotin-Kras[G12C] Protein, Human
Biotin-Kras[G12C] Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | GTPase KRas,KRAS2,RASK2 |
| Accession | P01116-2 |
| Amino Acid Sequence | M1-K169,with N-terminal 8x His tag-TEV, C-terminal Avi |
| Expression System | E.coli |
| Molecular Weight | 23.6 kDa |
| Purity | >90% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 10% glycerol, pH 7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
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