ALPP His Tag Protein, Human
ALPP His Tag Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | PLAP, PPB1, ALPI |
| Accession | P05187 |
| Amino Acid Sequence | Ile23-Asp506, with C-8*His |
| Expression System | HEK293 |
| Molecular Weight | PLAP, PPB1, ALPI |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
Alkaline phosphatase (ALPP) is plasma membrane bound glycoprotein involved in energy production. Human ALKP is classified into four tissue specific forms or isozymes, according to the site where it is expressed: placenta, intestine, liver, bone, kidney and germ cell. During pregnancy, serum ALPP levels increase progressively, reaching its highest value in the third trimester, mostly due to addition of placental isoenzyme. This usually results in doubling in the total ALPP level.
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SDS-PAGE
ELISA
Immobilized ALPP His Tag Protein, Human (Cat. No. UA010202) at 2.0μg/mL (100μL/well) can bind PLAP+ALPI Recombinant Rabbit mAb (SDT-167-155) (Cat. No. S0B2281) with EC50 of 0.60-0.87 ng/ mL.
