Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is less than 1ng/ml.
TPO (335aa) Protein, Mouse
TPO (335aa) Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Thrombopoietin; C-mpl ligand (ML); Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor (MGDF); Myeloproliferative leukemia virus oncogene ligand; |
| Accession | P40226 |
| Amino Acid Sequence | Ser22Thr356, with C-terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | 72-95 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Science. 2018 Apr 6;360(6384):106-110. |
Background
Thrombopoietin (TPO) is the primary cytokine responsible for regulating platelet production by stimulating the proliferation and differentiation of megakaryocytes, the bone marrow cells that produce platelets. TPO binds to the thrombopoietin receptor (TPO-R or c-Mpl) on hematopoietic stem cells (HSCs) and megakaryocyte progenitors, activating signaling pathways such as JAK2/STAT5, PI3K/AKT, and RAF-1/MAPK, which promote platelet production and maintain hematopoietic stem cell populations.
TPO and its receptor agonists (TPO-RAs) are widely used to treat immune thrombocytopenia (ITP), chemotherapy-induced thrombocytopenia, and thrombocytopenia associated with chronic liver disease or myelodysplastic syndromes (MDS)37. Recombinant human TPO (rhTPO) and synthetic TPO-RAs (e.g., romiplostim, eltrombopag, avatrombopag) have shown efficacy in increasing platelet counts in clinical settings.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized TPO (335aa) Protein, Mouse (Cat. No. UA040417) at 0.5μg/mL (100μL/well) can bind Biotinylated Thrombopoietin R/TPO R His Tag Protein, Mouse with EC50 of 0.44-1.02ug/mL.
