Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is less than 0.2ng/ml.
TPO (174aa) Protein, Mouse
TPO (174aa) Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Thrombopoietin; C-mpl ligand (ML); Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor (MGDF); Myeloproliferative leukemia virus oncogene ligand; |
| Accession | P40226 |
| Amino Acid Sequence | Ser22-Phe195, with C-terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | 26-29 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Autoimmunity. 2012 Sep;45(6):476-84. |
Background
Thrombopoietin (TPO) is a glycoprotein hormone that plays a crucial role in the regulation of platelet production by stimulating the proliferation and differentiation of megakaryocytes. The 174 amino acid (aa) form of TPO is a key isoform that has been extensively studied for its biological activities. In mice, TPO is essential for maintaining normal platelet counts and has been used as a model to understand its functions in hematopoiesis and thrombopoiesis.
TPO (174aa) is a target for the development of therapeutic agents aimed at treating thrombocytopenia and other platelet disorders. Recombinant TPO analogs and TPO receptor agonists are being developed and tested for their efficacy in increasing platelet counts. Also, TPO levels can be used as a biomarker for certain hematological conditions. Assays and kits for measuring TPO in biological samples are being developed for diagnostic purposes.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
>95% as determined by SEC-HPLC.
ELISA
Immobilized TPO Protein(174aa), Mouse His Tag (Cat. No. UA040416) at 0.5μg/mL (100μL/well) can bind Biotinylated Thrombopoietin R/TPO R His Tag Protein, Mouse with EC50 of 0.69-1.00ug/mL.
