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PDGF-BB Protein, Human

PDGF-BB Protein, Human

Catalog Number: UA040014 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE
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Regular price $240 USD
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Product Details

Product Specification


Species Human
Accession P01127
Amino Acid Sequence

Ser82-Thr190

Expression System Yeast
Molecular Weight

33-40 kDa(Non-reducing)

Purity

>95% by SDS-PAGE

Endotoxin <0.2EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized Powder
Storage Buffer

10 mM Acetic acid, pH3.3

Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.

Background

Platelet-derived growth factor (PDGF) is a family of three disulfide-linked glycoprotein dimers of M.W. 29kDa-32kDa that arise from astochastic assembly of the homologous subunits, A-chain and B-chain, yielding the heterodimer PDGF-AB and homodimers PDGF-AA and PDGF-BB. The B-chain contains a cationic carboxyl terminus that has been implicated in the strong association of PDGF-BB with the cell surface and wound healing.

Picture

Western Blot

Primary antibody: Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb(S0B1093) at 1/5000 dilution

Lane 1: untreated NIH/3T3 whole cell lysate 20 µg

Lane 2: NIH/3T3 starve 18 hours, then treated with 50 ng/ml PDGF-BB(Competitor R) for 20 minutes whole cell lysate 20 µg

Lane 3: NIH/3T3 starve 18 hours, then treated with 50 ng/ml PDG-BB(UA040014) for 20 minutes whole cell lysate 20 µg

Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 68 kDa

Observed MW: 68 kDa

Bioactivity

Measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, EC50 < 3.0 ng/ml.

SDS-PAGE

2μg(R: reducing conditions, N: non-reducing conditions).

ELISA

Immobilized PDGF-BB Protein, Human (Cat. No. UA040014) at 1.0μg/mL (100μL/well) can bind PDGF R alpha/PDGFRA Fc Chimera Protein, Human (Cat. No. UA010718) with EC50 of 23.57-26.73ng/mL.

Immobilized PDGF-BB Protein, Human (Cat. No. UA040014) at 1.0μg/mL (100μL/well) can bind PDGF R alpha/PDGFRA His Tag Protein, Human (Cat. No. UA010720) with EC50 of 0.19-0.26μg/mL.

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