Measured in a cell proliferation assay using NIH 3T3 mouse fibroblast cells, the EC50 for this effect is less than 65ng/ml.
PDGF-AA Protein, Mouse
PDGF-AA Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Antigen | PDGF-AA |
| Synonyms | Platelet-Derived Growth Factor-AA, Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF |
| Accession | P20033-1 |
| Amino Acid Sequence | Ser87-Thr211 |
| Expression System | E.coli |
| Molecular Weight | 29kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Souza P, Kuliszewski M, Wang J, Tseu I, Tanswell AK, Post M. PDGF-AA and its receptor influence early lung branching via an epithelial-mesenchymal interaction. Development. 1995 Aug;121(8):2559-67. 2. Demaria M, Ohtani N, Youssef SA, Rodier F, Toussaint W, Mitchell JR, Laberge RM, Vijg J, Van Steeg H, Dollé ME, Hoeijmakers JH, de Bruin A, Hara E, Campisi J. An essential role for senescent cells in optimal wound healing through secretion of PDGF-AA. Dev Cell. 2014 Dec 22;31(6):722-33. |
Background
Platelet-derived growth factor (PDGF) is a family of three disulfide-linked glycoprotein dimers of M.W. 29kDa-32kDa that arise from astochastic assembly of the homologous subunits, A-chain and B-chain, yielding the heterodimer PDGF-AB and homodimers PDGF-AA and PDGF-BB. PDGF-AA and its receptor represent an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized PDGF-AA, Mouse (Cat. No. UA040104) at 2μg/mL (100μL/well) can bind PDGF R alpha/PDGFRA His Tag, Mouse (Cat. No. UA010714) with EC50 of 0.25-0.34 μg/mL.
