Measured in a cell proliferation assay using murine MC/9 cells. The EC50 this effect is less than 2ng/ml.
IL-10 Protein, Human
IL-10 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IInterleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10; RP11-262N9.1; IL10A; MGC126450; MGC126451; TGIF |
| Accession | P22301 |
| Amino Acid Sequence | Ser19-Asn178 |
| Expression System | E.coli |
| Molecular Weight | 19kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH6.5 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1. Kim JM, Brannan CI, Copeland NG, et al. 1992. J Immunol. 148:3618-23. 2. Tan JC, Braun S, Rong H, et al. 1995. J Biol Chem. 270:12906-11. 3. Pestka S, Krause CD, Sarkar D, et al. 2004. Annu Rev Immunol. 22:929-79. |
Background
Interleukin 10(IL10), also known as cytokine
synthesis inhibitory factor (CSIF),is a secreted protein and belongs to the
IL-10 family. IL-10 is secreted by many activated hematopoietic cell types as
well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts .
IL-10 is an anti-inflammatory TH2 cytokine that has a critical role in limiting
the immune response to pathogens to prevent host damage. As IL-10 in produced
in several T helper populations, it is proposed that it provides a feedback
loop to limit the effector functions of macrophages and DCs on T cells. Once
expressed, IL-10 signals through the IL-10 receptor (IL-10R) to activate STAT3.
As IL-10 is a strong inhibitor of inflammation, it has become a viable
biomarker for various diseases and conditions as well as a therapeutic molecule
for certain conditions. In addition to elevated levels in parasitic infection,
high expression levels of IL-10 are also found in retroviral infections
inducing immunodeficiency. The immunosuppressive properties of IL-10 suggest a
possible clinical use of IL-10 in suppressing rejections of grafts after organ
transplantations.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized IL-10, Human (Cat. No. UA040176) at 5.0μg/mL (100μL/well) can bind IL10RA/CD210 His Tag, Human (Cat. No. UA010657) with EC50 of 13.88-19.80 ng/mL.
