| Species | Human |
| Synonyms | Leukocyte interferon. B cell interferon, Type I interferon, IFNA2, IFN-a 2a |
| Accession | P01563 |
| Amino Acid Sequence | Cys24-Glu188 |
| Expression System | E.coli |
| Molecular Weight | 17 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 150mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Cytokine. 2011 Jan;53(1):52-9. |
IFN-α is a cytokine that has an immunomodulatory function. It plays an important role not only in antiviral activity but also in several physiologic functions, such as activation of dendritic cells and accelerated expression of major histocompatibility complex I and II molecules that may cause increased antigen presentation. Variants of human leukocyte interferon α 2 (IFN-α 2a, α 2b, and α 2c) differ from each other by changes in their coding regions at nucleotide positions 137 and 170. IFN-α 2a is an important cytokine and used for antiviral and anticancer treatment.
Primary antibody: IFIT1 Recombinant Rabbit mAb (S0B1242) at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 ng/ml IFN-α 2a(UA040038) for 16 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 58 kDa
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The EC50 for this effect is less than 0.2 ng/ml.
Measured by a cytotoxicity assay using TF-1 Cells. The EC50 for this effect is less than 1ng/ml.
2μg (R: reducing condition, N: non-reducing condition).
>95% determined by RP-HPLC.
Protein A Chip captured IFN-alpha/beta R1 Fc Chimera Protein, Human (Cat. No. UA011186), can bind IFN-α 2a Protein, Human (Cat. No. UA040038) with an affinity constant of 1.30μM as determined in SPR assay.
