| Species | Human |
| Synonyms | Interferon-α1b, IFN-α 1b, Interferon alpha-1/13 |
| Accession | P01562 |
| Amino Acid Sequence | Cys24-Glu189 |
| Expression System | E.coli |
| Molecular Weight | 18-19 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 150mM NaCl, 1mM TCEP, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Blood. 2021 Sep 30;138(13):1162-1171. |
IFN-α is a cytokine that has an immunomodulatory function. It plays an important role not only in antiviral activity but also in several physiologic functions, such as activation of dendritic cells and accelerated expression of major histocompatibility complex I and II molecules that may cause increased antigen presentation. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Among the 13 subtypes of human IFNalpha, IFNalpha-1 subtype has 2 variants, named IFN-α 1a and IFN-α 1b, that differ from each other in only 1 amino acid, at residue 114. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. IFN-α is upregulated in preeclamptic placentas and is thought to be a mediator of preeclampsia.
Primary antibody: IFIT1 Recombinant Rabbit mAb (S0B1242) at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 ng/ml IFN-α 1b(UA040067) for 16 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 58 kDa
2μg (R: reducing condition, N: non-reducing condition).
Protein G Chip captured IFNAR1 Fc Chimera Protein, Human (Cat. No. UA011167) can bind IFN-α 1b Protein, Human (Cat. No. UA040067) with an affinity constant of 95.80nM as determined in SPR assay.
