Measured in a cell proliferation assay using Balb/3T3 mouse fibroblast cells, the EC50 for this effect is less than 0.1ng/ml.
FGF-basic(146aa) Protein, Human
FGF-basic(146aa) Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | bFGF,FGF-2;Heparin-binding growth factor 2;Basic fibroblast growth factor，FGFB,HBGF-2;FGF2; Fibroblast Growth Factor 2 (Basic) |
| Accession | P09038 |
| Amino Acid Sequence | Pro143-Ser288 |
| Expression System | E.coli |
| Molecular Weight | 17 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 500mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1. J Biol Chem. 1996 Jun 21;271(25):15292-7. |
Background
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
ELISA
Immobilized FGF-basic(146aa) Protein, Human (Cat. No. UA040145) at 2.0μg/mL (100μL/well) can bind Glypican 1/GPC1 His Tag Protein, human (Cat. No. UA011160) with EC50 of 18.49-26.03ng/mL.
Immobilized FGF-basic(146aa) Protein, Human (Cat. No.UA040145) at 2.0μg/mL (100μL/well) can bind GPC3 Fc Chimera Protein, Human (Cat. No. UA010680) with EC50 of 4.99-7.73 ng/mL.
Immobilized FGF-basic(146aa) Protein, Human (Cat. No. UA040145) at 2.0μg/mL (100μL/well) can bind GPC3 His Tag Protein, Human (Cat. No. UA010073) with EC50 of 21.65-30.89 ng/mL.
