Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The EC50 this effect is less than 0.2μg/ml in the presence of 2μg/ml recombinant Klotho β and 20μg/ml heparin.
FGF-21 Protein, Rat
FGF-21 Protein, Rat
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Product Details
Product Details
Product Specification
| Species | Rat |
| Synonyms | Fibroblast growth factor 21 |
| Accession | Q8VI80 |
| Amino Acid Sequence | Ala29-Ser208 |
| Expression System | E.coli |
| Molecular Weight | 20kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE&RP-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM PB,500mM NaCl,pH 7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. J Clin Invest. 2005 Jun;115(6):1627-35. . Epub 2005 May 2. |
Background
Fibroblast Growth Factor 21(FGF-21) is a member of the fibroblast growth factor (FGF) family and it is involved in the modulation of cell proliferation, growth, and differentiation as well as cell metabolism. This protein is a secreted endocrine factor that functions as a major metabolic regulator, and stimulates the uptake of glucose in adipose tissue. FGF21 binds to FGFR1c complexed with β-Klotho expressed in adipocytes to activate the canonical FGF signaling pathway (ERK1/2) and the AMPK/PGC1α pathway. Therefore, FGF-21 is being pursued as a therapeutic target for diabetes and obesity.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
96.4%
